An Efficient and Cost Effective Protocol for In Vitro

An efficient and cost effective protocol for in vitro propagation of Pineapple (Ananas comosus var. Queen) has been developed. In the proliferation stage, agar based Murashige and Skoog (MS) media was supplemented with 3.0 mg/l benzyleaminopurine (BAP), 0.5 mg/l indole acetic acid (IAA) and 50 mg/l adenine sulphate as RBC design experiment. Two approaches were taken to reduce the chemical cost of micropropagation media. Analytical grade sucrose was successfully replaced by commercial sugar, completely during proliferation stage and up to 66% during rooting stage. Again during the rooting stage, agar based solid media was replaced by liquid media (MSmedia). Bio-degradable Coir and Luffa were used as supporting matrix. As supporting matrix in rooting media, Luffa was found to be more effective. The clonal fidelity of in vitro raised plantlets was confirmed by RAPD technique.