Convenient and sensitive chemiluminescent detection system for 2-furancarboxylic acid using a continuous-flow method

A sensitive and convenient detection system for 2-furancarboxylic acid (2-FA) was developed using a three-channel continuous-flow manifold with chemiluminescence (CL) detection. The CL reaction involves hydrogen peroxide, alkali, alcohol and 2-FA methyl ester. 2-FA is first esterified to methyl 2-furancarboxylate (2-FM) with 70% efficiency. Analytical figures of ment for 2-FM were as follows: detection limit, 1.0 × 10−6 M (signal-to-noise ratio = 2): determination range, 1.0 × 10−6-0.1 M; sampling frequency, 60 h−1; and reproducibility (n = 10), 4.1% R.S.D, for l × 10−6 M 2-FM. A correlation between the present method and a gas Chromatographie method as a standard was good with a correlation coefficient of 0.999 (n = 5). The selectivity towards 2-FA compared with the related species, e.g., furyl methyl ketone, was also good. The recovery for normal human sera was 97–110%.