New percutaneous absorptiometry by a laser photoacoustic method using an open-ended cell

An UV laser photoacoustic (PA) method with high sensitivity and accuracy using a novel open-ended cell was developed. It was constructed by processing the end of a light guide as the sample chamber of the PA cell and by attaching an acoustic pipe bent at an optimized right angle to one end. A series of in vitro and in vivo percutaneous absorptiometric studies was performed using an anti-inflammatory agent and an anti-itching agent. As a result, it was concluded that this PA method was applicable to percutaneous absorption.