Peroxidase-incorporated hydrophilic polythiophene electrode for the determination of hydrogen peroxide in acetonitrile

Peroxidase-incorporated conducting polymer electrodes are examined as hydrogen peroxide sensors in acetonitrile. An aqueous solution containing horseradish peroxidase (HRP) and chemically polymerized poly(3-(3ʹ-thienyl)propanesulfonic acid) (PTS) was cast on SnO2-coated glass plates to obtain HRP/PTS electrodes. The electrode exhibits cathodic current responses to hydrogen peroxide (≥ 1 μM) at +100 to +700 mV vs. AgAg+ in acetonitrile containing 4% water and 0.1 M supporting salt (NaClO4 or tetra-n-butylammonium perchlorate).