Flow-injection chemiluminometric determination of ascorbic acid based on its sensitized photooxidation

The chemiluminescent reaction, in alkaline solution, of lucigenin with the products from the photooxidation of ascorbic acid sensitized by toluidine blue has been studied. A flow-injection configuration is proposed for the determination of ascorbic acid over a concentration range of 1 × 10−9 −3 × 10−4 M with a throughput of 80 samples/h. The method has been used for the simple and rapid determination of ascorbic acid in pharmaceuticals, fruit juices, soft drinks and blood serum.