Enhanced chemiluminescence kinetic ELISA of dichlorprop methyl ester

A competitive enzyme-linked immunosorbent assay (ELISA) for dichlorprop methyl ester (DME) with an anti-rabbit secondary antibody conjugated to peroxidase was developed in polystyrene plates. The plate was developed with a luminol-H2O2-horseradish peroxidase-p-iodophenol mixture by using a fibre optic to collect the chemiluminescent emission. The standard curve was produced for 0.05 to 10 000 ng ml−1 DME. The minimum detectable concentration was 0.11 ng ml−1 and the relative standard deviation was 7.26% for a 50 ng ml−1 sample of (n = 10). The ELISA procedure was selective with respect to structurally similar compounds usually found in formulations of pesticides. This method was applied to DME added to wheat grain extracts with recoveries between 97.1 and 103.6% for 5–500 ng ml−1.