Application of computerized compensation method to derivative synchronous spectrofluorimetry. Analysis of floctafenine and floctafenic acid in plasma

A compensation derivative synchronous spectrofluorimetric method has been developed for the simultaneous determination of floctafenine (F) and its major metabolite floctafenic acid (FA) in plasma samples. A study of the solvent effects and wavelength scanning intervals on the fluorescence spectra was carried out. The spectra of F and FA were partially resolved by synchronous spectrofluorimetry, in combination with the first-derivative measurements. Data manipulation was performed with a computer BASIC program to evaluate the so-called compensation balance point. Plasma samples were purified by deproteinization using a selected organic solvent prior to instrumental determination. The range of application was between 0.4 – 2.0 and 3 – 10 μg ml−1 plasma for F and FA, respectively. The overall recoveries of both compounds and the standard deviation data were reported. Day-to-day precision studies were carried out.