Rapid spectrofluorimetric method for traces of gibberelin

A new method for determination of gibberelin has been developed, based on the formation of a fluorescent compound with perchloric acid at 100 °C for 6 min. The maximum excitation and emission wavelengths of the fluorescent compound are 416 nm and 457 nm, respectively. Gibberelin in the range 10–800 ng ml−1 can be determined; the 3σ detection limit is 5 ng ml−1. The recommended method is very sensitive, selective, simple and rapid, making it ideal for the routine determination of gibberelin. The fluorescence reaction mechanism is discussed.