A Selective Sensor for Determination of Sitagliptinphosphate in Pharmaceutical Formulation

A selective electrode was developed for determination of sitagliptin using precipitation based technique with ammonium reineckate as anionic exchanger in polyvinyl chloride matrix. Linear responses of 1×10?5 to 1×10?2 M with slope of 40.9 mV/decade within pH 4-7. The percentage recovery for determination of sitagliptin by the proposed sitagliptin selective electrode was 100.06±1.15. Determination of sitagliptin in its pharmaceutical formulation by the proposed electrode revealed its applicability for its determination. The proposed method was compared with a reported one. No significant difference for both accuracy and precision was observed. The electrode exhibit good selectivity for sitagliptin with respect to a large number of inorganic, organic cations, sugars and amino acids. The proposed electrode offers the advantages of simplicity, accuracy and applicability to turbid and colored samples. The fabricated sensor was validated according to the International Conference on Harmonization (ICH) guidelines and successfully applied for the determination of the studied drug in pure form and pharmaceutical formulation without any interference.