Real-time simplex optimization of flow-injection systems for chemical analysis Original Research Article

A computer controlled flow-injection system was assembled to implement the real-time simplex algorithm. Two experimental variables, the flow rate and the sample-to-reagent ratio were controlled by a fully automated manifold. A computer interface was programmed to command the pumping speed, the introduction of sample-to-reagent amounts by solenoid valves, the on-time data acquisition and examination of the detector responses for application of the simplex algorithm. The feasibility of the approach was assessed by optimizing the flow parameters for chloride determination in water samples presenting different analyte concentrations. Optimum experimental conditions for chloride concentrations of 5.00, 25.0, 50.0 and 100 mg l−1 were maintained. A precision characterized by rsd lower than 1% and a sample throughput of 120 h−1 were attained.