Flow injection chemiluminometric determination of albumin Original Research Article

A new, simple analytical procedure for the determination of 5.00–300 μg ml−1 of albumin is presented, based on the chemiluminogenic reaction of the analyte with potassium permanganate in the presence of polyphosphoric acid (PPA). Serum globulins are also chemiluminescent, but their interference was eliminated by precipitation with saturated ammonium sulfate solution. The low limit of detection (4 μg ml−1) allows extensive dilution of the samples after globulin precipitation so that no other interferences are observed. Relative standard deviations were in the range 0.4–3.8% and the method was applied to the determination of albumin in human sera with good agreement with the bromcresol green (BCG) spectrophotometric method.