High-performance liquid chromatography of fatty acids in biological samples Review Article

This paper provides a comprehensive review of high-performance liquid chromatography (HPLC) methods for fatty acid analysis in various sample matrices. After a brief introduction about the generalities of HPLC, including its modes and applications, the review focuses on various aspects of fatty acid analysis by HPLC, such as sample preparation, mobile phases, stationary phases, detection techniques and the main matrices were fatty acids can be accessed, emphasizing the importance of this analysis in biological samples.