Studies of polyluminol modified electrode and its application in electrochemiluminescence analysis with flow system Original Research Article

In this work the electrochemical behavior of luminol in acidic solution is studied for the first time, and it is seen that luminol oxidized by electrochemistry can form the polymer on the surface of electrode, which is characterized by cyclic voltammetry (CV) and the surface enhanced Raman scattering (SERS) spectra. The mechanism of polymerization of luminol on electrode is also discussed. Furthermore, it is found that this modified electrode can greatly enhance the electrochemiluminescence (ECL) of flavin in Na2CO3–NaHCO3 buffer solution (pH 9.5). The selectivity for flavin is very high, most of the other water soluble vitamins and fluorescent compounds do not interfere, based on which a sensitive and selective ECL method for the determination of flavin is proposed. The linear range responding to flavin is 1.0×10−7–1.0×10−6 mol l−1, and the detection limit is 8.3×10−8 mol l−1.