On-line monitoring of nitric oxide complexed with porphyrine-bearing biochemical materials by using flow injection with chemiluminescence detection Original Research Article

An on-line nitric oxide (NO) monitoring method is described employing a flow injection manifold with chemiluminescence (CL) detection. The method is tailored for monitoring the NO from its complexes with porphyrin ring-bearing (PRB) biochemical materials. The method is optimized for sensitivity and detection limit. The method is quite sensitive, simple, rapid, and precise. A sensitivity of 2.63×105 mV mol−1 NO, a range of 2.0×10−5 up to 1.0×10−2 mol NO (under the conditions used) a reproducibility of 1.0% over the range 1.0×10−3 up to 1.0×10−2 mol NO, a frequency of ca. five samples per minute and a detection limit of 1.0×10−5 mol NO are the analytical figures of merit for the proposed method.