Indirect determination of superoxide anion radical in the plant of red sage based on vanillin-8-aminoquinoline with fluorescence Original Research Article

A novel spectrofluorimetric method using vanillin-8-aminoquinoline (VAQ) as fluorescent probe was developed for the determination of superoxide anion radical (O2radical dot−). The new fluorescent probe was characterized by elemental analysis and IR spectra. Under the optimum conditions of the determination, the linear calibration range and the detection limit of the developed method for superoxide anion radical were in the range (0.0–1.0)×10−5 and 2.0×10−8 mol l−1, respectively. The effect of interferences was studied. The proposed method was applied to determine the generation rate of superoxide anion radical in the course of aging in red sage successfully.