Induction of Callus and Somatic Embryogenesis from Cotyledon Explants of Fagonia indica Burm

To explore the potential for in vitro rapid regeneration of Fagonia indica Burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on MS medium which supplemented with 1.0-5.0 µM 2,4-D. Callus was initiated from cotyledon explant on MS medium supplemented in all concentrations of 2,4-D investigated. 2,4-D was more effective than IAA and NAA for callus induction, but auxins and cytokines’ combination is essential for the green callus induction for somatic embryogenesis. Induced calluses were subcultured on media containing 2.5-5.0 µM BA or Kin combined with different concentrations IAA, NAA and 2,4-D. Elimination of 2,4-D and a slight increase of BA concentration induced of the relating somatic embryogenesis. The maximum globular structure embryos were further enlarged and produced the somatic embryos in MS basal medium supplemented with 5.0 BA µM and also combined with either 2.5 µM NAA. Continued formation of the globular embryo occurred in these mediums for 12 months. The maximum germinations of embryos were observed in MS medium + 1.5% sucrose without growth regulators.