Novel Bioactive Steroidal Alkaloids from Pachysandra procumbens

(+)-(20S)-20-(Dimethylamino)-3α-(methylbenzoylamino)-11-methylene-5α-pregnane (1), a steroidal alkaloid with a new substitution pattern, was isolated from the entire plant of Pachysandra procumbens, together with four other new steroidal alkaloids, (+)-(20S)-3-(benzoylamino)-20-(dimethylamino)-5α-pregn-2-en-4β-ol (2), (+)-(20S)-20-(dimethylamino)-16α-hydroxy-3β-(3′α-isopropyl)-lactam-5α-pregn-4-one (3), (+)-(20S)-20-(dimethylamino)-3α-(methylbenzoylamino)-5α-pregn-12β-yl acetate (4), and (+)-(20S)-20-(dimethylamino)-3α-(methylsenecioylamino)-5α-pregn-12β-ol (5), as well as two known compounds, (+)-pachysamine H (6) and (+)-pachysandrine B (7). The structures of the new compounds were determined by spectroscopic methods. Compounds 1–7 were evaluated for their potential cancer chemopreventive properties using an in vitro estrone sulfatase assay.