Title of article
Cyclodextrin-assisted Glycan Chain Extension on a Protected Glycosyl Amino Acid
Author/Authors
Gregor Dudziak، نويسنده , , Nicole Bézay، نويسنده , , Tilo Schwientek، نويسنده , , Henrik Clausen، نويسنده , , Horst Kunz، نويسنده , , Andreas Liese، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2000
Pages
5
From page
5865
To page
5869
Abstract
By the use of cyclodextrins, we have enhanced the solubility of the protected amino acid glycan Fmoc-Thr(GalNAcα1)-OtBu (1b) up to 100-fold. This improvement enabled us to carry out an enzymatic glycosylation employing a β-galactosidase in combination with an α2,3-sialyltransferase without the aid of organic cosolvents. After optimization of the one-pot reaction, the sialylated core 1 structure Fmoc-Thr[Neu5Ac(α2-3)Gal(β1-3)GalNAcα1]-OtBu (3b) could be obtained with 50% yield.
Keywords
Biosynthesis , Cyclodextrins , Glycopeptides , Glycosidation
Journal title
Tetrahedron
Serial Year
2000
Journal title
Tetrahedron
Record number
1081082
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