Title of article
High Level Production, Characterization and Construct Optimization of the Ionotropic Glutamate Receptor Ligand Binding Core
Author/Authors
Guo Qiang Chen، نويسنده , , Eric Gouaux، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2000
Pages
11
From page
9409
To page
9419
Abstract
Multimilligram quantities of active and pure GluR2-S1S2, the recombinant ligand binding core of the AMPA-sensitive GluR2 receptor, were produced by preparative folding of the solubilized inclusion bodies expressed in 1 l of Escherichia coli cell culture. The biochemical properties and biological activities of folded protein were characterized and the protein construct was optimized for three-dimensional structural studies.
Keywords
Ligand binding , Escherichia coli , protein engineering , preparative folding , glutamate receptor , X-ray crystallography , protein production
Journal title
Tetrahedron
Serial Year
2000
Journal title
Tetrahedron
Record number
1081463
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