Title of article
Sequential native chemical ligation utilizing peptide thioacids derived from newly developed Fmoc-based synthetic method
Author/Authors
Akira Shigenaga، نويسنده , , Yoshitake Sumikawa، نويسنده , , Shugo Tsuda، نويسنده , , Kohei Sato، نويسنده , , Akira Otaka، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2010
Pages
7
From page
3290
To page
3296
Abstract
The first facile Fmoc-based synthetic procedure for peptide thioacids was developed. Successful application of the resulting thioacids to sequential native chemical ligation (NCL) in the N to C direction was achieved. Conversion of the peptide thioacids to the corresponding thioesters with Ellmanʹs reagent followed by NCL in the presence of tris(2-carboxyethyl)phosphine (TCEP) and thiophenol was accomplished in a one-pot manner.
Keywords
N-S Acyl transfer , Native chemical ligation , Fmoc solid-phase peptide synthesis , Peptide thioacids , Peptide thioesters
Journal title
Tetrahedron
Serial Year
2010
Journal title
Tetrahedron
Record number
1100783
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