On stabilization of a neutral aromatic ligand by π–cation interactions in monoclonal antibodies Original Research Article

It has been shown that anti-PAH mAb can bind a particular cross-reactant by adopting two distinct “red” and “blue” conformations of its binding sites [N.M. Grubor et al. PNAS 102, 2005, 7453–7458]. In the case of red conformation of pyrene (Py)/anti-PAH mAb (with a broad fluorescence (0,0)-band with fwhm ~ 140 cm−1), the central role in complex formation was played by π–π interactions. The nature of the blue-shifted conformation with very narrow fluorescence (0,0)-band (fwhm ~ 75 cm−1) was left unclear due to the lack of suitable data for comparison. In this work, we suggest spectroscopic and modeling results obtained for the blue conformation of Py in several mAb (including 4D5 mAb) are consistent with π–cation interactions, underscoring the importance of π–cation interaction in ligand binding and stabilization in agreement with earlier modeling studies [J-L. Pellequer, et al. J. Mol. Biol. 302, 2000, 691–699]. We propose considerable narrowing of the fluorescence origin band of ligand in the protein environment could be regarded as a simple indicator of π–cation interactions. Since 4D5 mAb forms only the blue-shifted conformation, while anti-PAH and 8E11 mAbs form both blue- and red-shifted conformations, we suggest mAb interactions, with Py molecules lacking H-bonding functionality, may induce distinct conformations of mAb binding sites that allow binding by π–π and/or π–cation interactions.