• Title of article

    An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate

  • Author/Authors

    Sunita Teotia، نويسنده , , S.K. Khare، نويسنده , , M.N. Gupta، نويسنده ,

  • Issue Information
    روزنامه با شماره پیاپی سال 2001
  • Pages
    4
  • From page
    792
  • To page
    795
  • Abstract
    β-amylases are used in production of maltose syrup. It is shown that sweet potato β-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of β-amylases.
  • Keywords
    Alginate , Enzyme purification , ?-amylase , Affinity precipitation , Sweet potato
  • Journal title
    Enzyme and Microbial Technology
  • Serial Year
    2001
  • Journal title
    Enzyme and Microbial Technology
  • Record number

    1173443