Title of article
An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate
Author/Authors
Sunita Teotia، نويسنده , , S.K. Khare، نويسنده , , M.N. Gupta، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2001
Pages
4
From page
792
To page
795
Abstract
β-amylases are used in production of maltose syrup. It is shown that sweet potato β-amylase can be purified by affinity precipitation with alginate with 80% activity yield and 44 fold purification. SDS-PAGE of the purified protein showed a single band and a subunit weight of 50 kDa. Preliminary data with soybean and barley enzymes indicate that this may be a general method for purification of β-amylases.
Keywords
Alginate , Enzyme purification , ?-amylase , Affinity precipitation , Sweet potato
Journal title
Enzyme and Microbial Technology
Serial Year
2001
Journal title
Enzyme and Microbial Technology
Record number
1173443
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