Immobilization of dextransucrase on regenerated benzoyl cellulose carriers

The regeneration of the benzoyl cellulose carrier was accomplished by treatment by Triton X-100, sodium dodecyl sulfate (SDS), or alkyldimethylbenzyl-ammonium chloride (ADMB) detergent solutions, or by NH4SCN as chaotropic agent. It was found that Triton X-100, as a more hydrophobic substance, was linked to the carrier, eluting the protein from the carrier. Regenerated in this way, the carrier very efficiently binds new amounts of proteins, including dextransucrase, without regarding the fact that Triton X-100 was washed out by alcohol, or remained linked to the carrier. Dextransucrase immobilization at this regenerated carriers yielded high values for the immobilized activity, total preserved activity and immobilization efficiency.