Title of article
Expression and characterization of the nitrile reductase queF from E. coli
Author/Authors
Kathrin Moeller، نويسنده , , Giang Son Nguyen، نويسنده , , Frank Hollmann، نويسنده , , Ulf Hanefeld، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
5
From page
129
To page
133
Abstract
The expression and characterization of a nitrile reductase from Escherichia coli K-12 (EcoNR), a newly discovered enzyme class, is described. This enzyme has a potential application for an alternative nitrile reduction pathway. The enzyme activity towards its natural substrate, preQ0, was demonstrated and optimal working conditions were found to be at 37 °C and at pH 7 with Tris buffer.
Keywords
Nitrile reductase , nitriles , Activity assay , Substrate screening , Enzymatic reduction , Amines
Journal title
Enzyme and Microbial Technology
Serial Year
2013
Journal title
Enzyme and Microbial Technology
Record number
1185982
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