Nanoceria: Gum mediated synthesis and in vitro viability assay

Green and Biological synthetic methods for preparing of different nanoparticles (e.g., metal and metal oxide) using microorganisms, enzymes, and plant extracts have been suggested as a possible low cost and eco-friendly alternative to chemical and physical methods. In this paper, we would like to report the green and biosynthesis of nanoceria particles (NCs), using gum tragacanth by both chemical and biological methods. The NCs were synthesized at different calcination temperatures and structural, morphological, and optical properties of the synthesized NCs have been characterized by using UV–vis spectrophotometer, FESEM, PXRD analysis. FESEM images display that the NCs prepared were mono-dispersed and the average size was ranged from about 20 to 40 nm. In vitro viability studies on neuro2A cells, a dose dependent toxicity with non-toxic effect of concentrations below 30 µg/mL is presented. The synthesis of NCs using gum tragacanth was found to be comparable to those obtained from conventional reduction methods using hazardous polymers or surfactants.