Effects of chirality and substituents at carbon 3 in dihydroxyacetone-phosphate analogues on their binding to Rabbit muscle aldolase Original Research Article

A series of dihydroxyacetone-phosphate (DHAP) analogues has been synthesized, differing in their stereochemistry and functionality at C-3. The kinetic effects of these compounds on the enzyme aldolase (EC 4.1.2.13) have been studied and differing modes of action observed. Competitive and time dependant reversible inhibition have been shown to take place both with and without borohydride detected formation of an immonium ion.