Convenient chemoenzymatic synthesis of β-purine-diphosphate sugars (GDP-fucose-analogues) Original Research Article

A series of peracetylated β-sugar-1-phosphates with l-fuco configuration are efficiently prepared chemically and coupled in high yield to purine monophosphate bases via imidazolide activation. The resulting purine diphosphate sugars are deacetylated completely by a mild treatment with commercial acetylesterase (EC 3.1.1.6) to give donor-substrates for fucosyltransferases.