Ptaquiloside Z, a new toxic unstable sesquiterpene glucoside from the neotropical bracken fern pteridium aquilinum var. caudatum Original Research Article

Reversed-phase HPLC fractionation, monitored by brine shrimp bioassay, led to the isolation of a new illudane-type sesquiterpene glucoside, ptaquiloside Z (2), as well as the known bracken carcinogen ptaquiloside (1), from a bioactive aqueous extract of the neotropical bracken fern Pteridium aquilinum var. caudatum (Pteridaceae). The structure of ptaquiloside Z (2) was confirmed by spectroscopic analyses and chemical degradation. Both compounds exhibited similar toxicity (LC50 62.5 μg/ml at 24 h and LC50 7.8 μg/ml at 48 h) toward brine shrimp.