Site-specific incorporation of the 1-hexanol-1,N6-etheno-2′-deoxyadenosine adduct into oligodeoxyribonucleotides Original Research Article

Modified oligonucleotides that contain the hydrophobic 1-hexanol-1,N6-etheno-2′-deoxyadenosine adduct have been synthesized using a mild solid phase phosphoramidite chemistry. The presence and the integrity of the modified nucleoside in the synthetic oligomers were confirmed by electrospray ionization and MALDI mass spectrometry measurements together with analysis of the complete enzymatic hydrolysate by high performance liquid chromatography coupled to UV and fluorescent detection techniques.