Title of article
Pyrene–perylene as a FRET pair coupled to the N2′-functionality of 2′-amino-LNA Original Research Article
Author/Authors
Dorthe Lindegaard، نويسنده , , Andreas S. Madsen، نويسنده , , Irina V. Astakhova، نويسنده , , Andrei D. Malakhov، نويسنده , , B. Ravindra Babu، نويسنده , , Vladimir A. Korshun، نويسنده , , Jesper Wengel، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
6
From page
94
To page
99
Abstract
Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.
Keywords
Perylene , Amino-LNA , Pyrene , FRET
Journal title
Bioorganic and Medicinal Chemistry
Serial Year
2008
Journal title
Bioorganic and Medicinal Chemistry
Record number
1303882
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