Title of article
Characterization of the full length coat protein gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis
Author/Authors
Sokhandan Bashir، Nemat نويسنده Plant Protection Department, University of Tabriz, P.O. Box 345, Tabriz, I.R. Iran , , Pashaei، Alireza نويسنده Plant Protection Department, University of Tabriz, P.O. Box 345, Tabriz, I.R. Iran , , Doulati Baneh، Hamed نويسنده ,
Issue Information
فصلنامه با شماره پیاپی 0 سال 2011
Pages
9
From page
213
To page
221
Abstract
The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized by reverse transcription polymerase chain reaction (RT-PCR) and sequencing. The expected 1515 bp coat protein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibody sandwich enzyme-linked immunesorbent assay (DAS-ELISA) in a population of 330 symptomatic grapevine leaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined. Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.
Journal title
Iranian Journal of Biotechnology (IJB)
Serial Year
2011
Journal title
Iranian Journal of Biotechnology (IJB)
Record number
1314551
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