Novel electron transfer mediators, indoaniline derivatives for amperometric lactate sensor

Novel indoaniline derivatives were synthesized and characterized electrochemically with respect to their abilities to act as electron transfer mediators for lactate oxidase (LOD). A lactate sensor using these derivatives demonstrated the clear independence on oxygen, a result which can never be achieved by H2O2 detecting system and the low interference effects. This sensor could achieve the excellent durability which maintained the >30% of the original activity and good linearity in the range from 0 to 16 mM lactate concentration until day 19.