Changes in endogenous cellulase gene expression levels and reproductive characteristics of primary and secondary reproductives with colony development of the termite Reticulitermes speratus (Isoptera: Rhinotermitidae)

The unique feature of eusociality is reproductive division of labor. At the termite colony initiation stage, there are only reproductives plus larvae and eggs that need to be cared for. As workers increase in number with colony development, reproductives are thought to be relieved of the cost of brood care and able to resume reproduction. This transition of responsibility for the care of offspring from parents to their offspring has been considered as a crucial step in the evolution of termite eusociality (trophic shift model). Here, we studied the endogenous cellulase gene expression levels and reproductive characteristics of alates and primary reproductives at 30, 50, 100, and 400 days after colony foundation in the termite Reticulitermes speratus. As a result, when the numbers of workers reached about 100 (400 days), wood digestion abilities of alates and primary reproductives decreased significantly while ovaries and testis were well developed. Moreover, wood digestion abilities of female secondary reproductives with well-developed ovaries, collected in the field colony, were lower than those of any stages of primary queens. These results were consistent with the trophic shift model, and suggested the importance of switch from parental care to alloparental care for reproductive division of labor and evolution of termite eusociality.