Towards parallelized surface plasmon resonance sensor platform for sensitive detection of oligonucleotides

We report a new multichannel biosensor combining an optical platform based on surface plasmon resonance (SPR) imaging on special multilayers and polarization contrast with a spatially-resolved functionalization. We demonstrate that the optical platform offers a considerably higher sensitivity and resolution than the conventional SPR imaging. The spatially-resolved functionalization based on microspotting applied to immobilization of short oligonucleotides is shown to provide a surface concentration of oligonucleotide probes higher by 80% than the flow-through functionalization method. The sensor can perform 64 independent measurements simultaneously and its limit of detection (LOD) for 23-mer oligonucleotides was demonstrated to be as low as 100 pM.