Title of article
Novel optimized biofunctional surfaces for Love mode surface acoustic wave based immunosensors
Author/Authors
Dinh، نويسنده , , Duy Hai and Pascal، نويسنده , , ةmilie and Vellutini، نويسنده , , Luc and Bennetau، نويسنده , , Bernard and Rebière، نويسنده , , Dominique and Dejous، نويسنده , , Corinne and Moynet، نويسنده , , Daniel and Belin، نويسنده , , Colette and Pillot، نويسنده , , Jean-Paul، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2010
Pages
8
From page
289
To page
296
Abstract
A very-long-chain (C22) organosilicon self-assembled monolayer (SAM) with terminal glycidyl groups was used to immobilize antibodies on the silica waveguide of a Love mode surface acoustic wave (SAW) immunosensor. This route enabled us to specifically detect, in real time, living Escherichia coli bacteria by using monoclonal anti-E. coli antibodies, leading to a marked sensor response in liquid aqueous medium. Conversely, no bacteria detection could be observed by this direct method upon functionalization of the waveguide surface by a (3-glycidoxypropyl) trimethoxysilane (GPTS) monolayer. This improvement was attributed to a surface smoothing effect resulting from the very-long-chain SAM and a better accessibility of the surface epoxide groups.
Keywords
SAW immunosensor , antibodies , Biofunctionalization , Very-long-chain glycidyl-terminated SAM , E. coli , Surface smoothing
Journal title
Sensors and Actuators B: Chemical
Serial Year
2010
Journal title
Sensors and Actuators B: Chemical
Record number
1438167
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