Surface plasmon resonance imaging based multiplex biosensor: Integration of biomolecular screening, detection and kinetics estimation

We present a multiplex biosensing method to simultaneously screen targets of interest in a multiple target analyte sample and to extract the binding affinities of all interactant pairs from a single sensor surface using a commercial surface plasmon resonance imaging system. For demonstration, we have prepared our sensor disk with five different ligands varying from low molecular weight antibiotics to high molecular weight human IgG, all immobilized in a microarray format. The multi-target analyte sample was prepared by mixing five antibodies where each one is highly specific for one of the immobilized ligands in a range of concentrations for kinetics estimations. The key advantage of the newly developed approach is that many different types of assays can be performed simultaneously, however, care should be taken to understand the non-specific interactions between different analytes in the sample mixture and unintended ligands, and surface regeneration behavior of different ligand types. Other advantages include reductions in experimental and analysis time, reduced costs, and flexibility since the same microarray can be used for assays with a single target analyte specific for the single ligand.