Preparation and bioelectrochemical responses of the poly(m-phenylenediamine) glucose oxidase electrode

A new technique termed the “pulse galvanostatic method (PGM)” is described for constructing a glucose sensor by electropolymerization of m-phenylenediamine in the phosphate buffer solution containing m-phenylenediamine and glucose oxidase (GOx). The conditions for fabrication of the poly(m-phenylenediamine) glucose oxidase (PMPD GOx) electrode were optimized as follows: ton/toff, 1; frequency, 10; mean current density, 1 mA cm−2; concentration of the GOx, 5 g l−1. The morphology of PMPD GOx electrode prepared by PGM under the optimized conditions shows porous structure. The PMPD GOx electrode has short response time, high response current, good selectivity and stability. The maximum response current density and Michaelis–Menten constant of the PMPD GOx electrode, based on the calculation of the kinetic parameters, are 231.4 μA cm−2 and 15.33 mM, respectively.