SECM characterization of immobilised enzymes by self-assembled monolayers on titanium dioxide surfaces

SECM in generator-collector mode was used to detect the presence of immobilised enzymes on titanium dioxide layers which were chemically or electrochemically generated with possible application as chemical sensors and biosensors. Glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilised by SAM generation using aminopropyltriethoxysilane (APTES) and ascorbic acid. The enzymes were successfully immobilised on two different TiO2 surfaces. A simple test of durability of the system was made and a model of SAM organisation is presented.