The inhibition of Saccharomyces cerevisiae cells by acetic acid quantified by electrochemistry and fluorescence

In this paper, the effect of acetic acid on S. cerevisiae metabolism was studied for the first time with the combinational utilization of the mediated electrochemical method and intrinsic fluorescence method, and the results from both methods were compared with each other. It was confirmed in vivo that not only the glycolysis enzymes but also the NADH dehydrogenase were sensitive to acetic acid. The established technique might also be applicable in studying the toxicity of other environmental pollutants, such as phenol and its derivatives.