Determination of polychlorinated biphenyl congeners in human milk by gas chromatography–negative chemical ionization mass spectrometry after sample clean-up by solid-phase extraction

This paper describes a simple and efficient procedure for measuring 25 congeners of polychlorinated biphenyls in human milk. The limit of quantitation was 0.1 ng/ml for five less chlorinated congeners (PCB 70, 74, 87, 99,101), and 0.01 ng/ml for the remaining 20 congeners (PCB 77, 105, 118, 126, 128, 138, 151, 153, 156, 169, 170, 180, 183, 187, 191, 194, 205, 206, 208 and 209). Solid phase extraction technology was applied to extract the analytes from the matrix and to remove lipids. Three columns were used sequentially, and they were a Bond Elut C18, a Sep-Pak Plus NH2 and a Bond Elut PCB cartridge. The instrumental method was gas chromatography–mass spectrometry with negative chemical ionization, and selected ion monitoring mode was used.