Title of article
Quantitation of yeast ceramides using high-performance liquid chromatography–evaporative light-scattering detection
Author/Authors
Zhou، نويسنده , , Quan and Zhang، نويسنده , , Wei-lin and Fu، نويسنده , , Xue-Qi and Chen، نويسنده , , Guo-Qiang، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
9
From page
161
To page
169
Abstract
A high-performance liquid chromatograph equipped with an evaporative light scattering detector (ELSD) (HPLC–ELSD) was used to assay the ceramides in yeast cells. The HPLC–ELSD method employed a cyanopropyl bonded column (CN column) that effectively separated the main interfering substance ergosterol without any derivatization process; most other interfering substances were also removed. The method can be applied for routine assay of ceramide content in yeast.
Keywords
Ceramides
Journal title
Journal of Chromatography B
Serial Year
2002
Journal title
Journal of Chromatography B
Record number
1454283
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