Title of article
Single-based resolution for oligodeoxynucleotides and their phosphorothioate modifications by replaceable capillary gel electrophoresis
Author/Authors
Chen، نويسنده , , Rong and Luo، نويسنده , , Xuefang and Di، نويسنده , , Xin and Li، نويسنده , , Ying and Sun، نويسنده , , Yuqing and Hu، نويسنده , , Yuzhu، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2006
Pages
5
From page
334
To page
338
Abstract
A replaceable capillary gel electrophoretic (replaceable CGE) method was developed for the separation of two sets of model compounds of single-stranded oligodeoxynucleotide mixtures (18–20 mers), phosphodiester oligodeoxynucleotides (PO-ODNs) and their phosphorothioate modifications (PS-ODNs), with equal sequences differing in a single base. Polyethylene glycol (PEG) 35000 was chosen as the sieving matrix. It was confirmed that PEG polymer solution less influenced resolutions of the PS-ODNs compared with those of the PO-ODNs, while acetonitrile used as an additive in the system improved the separation significantly. It was also noticed that the effect of temperature on separation was much larger than that of denaturant urea.
Keywords
Single-based resolution , Phosphodiester oligodeoxynucleotide , Phosphorothioate modified oligodeoxynucleotide , Replaceable capillary gel electrophoresis
Journal title
Journal of Chromatography B
Serial Year
2006
Journal title
Journal of Chromatography B
Record number
1463564
Link To Document