Title of article
Relationship between MPA free fraction and free MPAG concentrations in heart transplant recipients based on simultaneous HPLC quantification of the target compounds in human plasma
Author/Authors
Cussonneau، نويسنده , , Xavier and Bolon-Larger، نويسنده , , Magali and Prunet-Spano، نويسنده , , Céline and Bastien، نويسنده , , Olivier and Boulieu، نويسنده , , Roselyne، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
5
From page
674
To page
678
Abstract
A simple and sensitive HPLC method for the simultaneous analysis of free MPA and free MPAG was developed. Separation was achieved on a X-Terra RP18 column with acetonitrile–40 mM orthophosphoric acid as eluents using a gradient elution mode over 35 min at a flow rate of 1.5 ml/min. The assay was linear in the range 0.005 mg/L (LOQ) to 5 mg/L for free MPA and 0.05 mg/L (LOQ) to 200 mg/L for free MPAG. Isolation of free MPA and free MPAG was done by ultrafiltration and the ultrafiltrate was directly injected. A positive correlation between MPA free fractions and free MPAG concentrations was found. Likewise, free MPAG was related to total MPAG concentrations in the seven heart transplant patients.
Keywords
HPLC , Mycophenolic acid , Free fraction , Ultrafiltration
Journal title
Journal of Chromatography B
Serial Year
2007
Journal title
Journal of Chromatography B
Record number
1464638
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