Title of article
Determination of andrographolide in human plasma by high-performance liquid chromatography/mass spectrometry
Author/Authors
Gu، نويسنده , , Yanli and Ma، نويسنده , , Jinyu and Liu، نويسنده , , Yali and Chen، نويسنده , , Bo and Yao، نويسنده , , Shouzhuo، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2007
Pages
4
From page
328
To page
331
Abstract
In this paper, a rapid method based on high-performance liquid chromatography/electrospray–mass spectrometry (HPLC/ESI-MS) method for the quantitative determination of andrographolide (AND) in human plasma has been developed and validated. A liquid–liquid extraction (LLE) procedure was selected to isolate AND from biological matrixes. Isosorbide-5-mononitrate (IS-5-MN) was selected as the internal standard (IS). The correlation coefficient of the calibration curve was 0.998, in the range of 9.9–320.0 ng/mL. The validated method may be used to assess the bioavailability and pharmacokinetics of the drug.
Keywords
HPLC/ESI-MS , Andrographolide , Human plasma
Journal title
Journal of Chromatography B
Serial Year
2007
Journal title
Journal of Chromatography B
Record number
1464866
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