Title of article
A novel method for the analysis of 3-mercaptopyruvate using high-performance liquid chromatography with fluorescence detection
Author/Authors
Ogasawara، نويسنده , , Yuki and Hirokawa، نويسنده , , Tomoaki and Matsushima، نويسنده , , Kaori and Koike، نويسنده , , Shin and Shibuya، نويسنده , , Norihiro and Tanabe، نويسنده , , Shinzo and Ishii، نويسنده , , Kazuyuki، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2013
Pages
5
From page
56
To page
60
Abstract
3-Mercaptopyruvate (3-MP) is a metabolite of cysteine present in mammalian tissues and is known to be a substrate of 3-mercaptopyruvate sulfurtransferase (3MST, EC.3.4.1.2). The physiological relevance of the 3-MP pathway has not been fully recognized because the metabolic behavior of 3-MP remains unclear. Here, we describe a novel method using high-performance liquid chromatography with fluorescence detection to measure 3-MP formation from cysteine. To demonstrate the practical value of the present method, we applied it to analyze the 3-MP produced in biological samples from mouse tissue.
Keywords
3-Mercaptopyruvate , Cysteine metabolism , Hydrogen sulfide , Bound sulfur , Sulfurtransferase
Journal title
Journal of Chromatography B
Serial Year
2013
Journal title
Journal of Chromatography B
Record number
1471267
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