Comparison of the thin-layer chromatographic properties of sulfur-containing amino acids and their aminophosphonic analogues

The thin-layer chromatographic behavior of the natural, sulfur-containing amino acids (Cys, Hcys, Met) and their phosphonic analogues (CysP, HcysP, MetP) has been studied. Their detection limits using iodine and molybdate, ninhydrine and iodine–azide reagents were determined, and the RF values of these two classes of amino acids in acidic, neutral and mild basic solvent systems were compared.