Polymer characterization by size-exclusion chromatography with multiple detection

Size-exclusion chromatography with coupled multiangle light scattering and differential refractometry detectors has been used to obtain molecular mass and radius of gyration distributions of polydisperse polymer samples. From these data the scaling law between dimensions and the absolute molecular mass is obtained with just one sample of each polymer. Three different kinds of polymers are presented: polystyrene which serves as reference polymer, polyphosphazenes which behave abnormally in solution and poly(ethylene oxide) which is soluble in water. Since the relationship between dimensions and molecular mass depends on the extent of interactions between chain segments and solvent molecules, the scaling law provides information about the solution properties of the polymer.