Normal-phase high-performance liquid chromatographic separations using ethoxynonafluorobutane as hexane alternative: I. Analytical and chiral applications

A novel, environmentally friendly, fluorinated solvent — ethoxynonafluorobutane — has been used to replace n-hexane in normal-phase HPLC applications. Fast gradients of methanol in ethoxynonafluorobutane on a cyano column have been successfully applied to the separation of steroids, benzodiazepines, NSAIDs, tricyclic antidepressants, β-adrenergic blocking agents and mixtures of purines and pyrimidines. Small amounts of triethylamine and trifluoroacetic acid added to such gradients significantly improved peak shape and column performance for basic and acidic solutes. Ethoxynonafluorobutane and its mixtures with methanol have also been demonstrated to have a unique selectivity in chiral HPLC applications.