Title of article
Immunoaffinity chromatography for the sample pretreatment of Taxus plant and cell extracts prior to analysis of taxanes by high-performance liquid chromatography
Author/Authors
Theodoridis، نويسنده , , G and Haasnoot، نويسنده , , W and Cazemier، نويسنده , , G and Schilt، نويسنده , , R and Jaziri، نويسنده , , M and Diallo، نويسنده , , B and Papadoyannis، نويسنده , , I.N and de Jong، نويسنده , , G.J، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2002
Pages
9
From page
177
To page
185
Abstract
The application of immunoaffinity chromatography for the purification of Taxus plant and cell extracts prior to the HPLC analysis is described. Polyclonal antibodies raised against 10-deacetylbaccatin III (10-DAB III), paclitaxel’s main precursor in plant, were characterised by enzymed-linked immunosorbent assay. Immunoglobulins from selected antisera were immobilised on CNBr-activated Sepharose 4B. The immunoaffinity column was used for the purification of plant and plant cell culture extracts prior to their analysis by HPLC. Immunoaffinity chromatography enabled the selective concentration of taxoids and enhanced sample clean-up.
Keywords
taxol , Deacetylbaccatin III , Taxanes , Paclitaxel
Journal title
Journal of Chromatography A
Serial Year
2002
Journal title
Journal of Chromatography A
Record number
1510201
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