Profiling of components and validated determination of iridoids in Gardenia Jasminoides Ellis fruit by a high-performance-thin-layer- chromatography/mass spectrometry approach

A novel method was set up with the aim to obtain a simultaneous cross comparative evaluation of different Gardenia Jasminoides Ellis fruits by the HPTLC fingerprint approach. The main components among the iridoid, hydroxycinnamic derivative and crocin classes were identified by TLC–MS ancillary techniques. The iridoids geniposide, gardenoside and genepin-1-β-d-gentiobioside were also quantitated by densitometric scanning at 240 nm. LiChrospher HPTLC Silica gel 60 RP-18 W F254, 20 cm × 10 cm plates with acetonitrile: formic acid 0.1% (40:60 v/v) as the mobile phase was used. thod was validated giving rise to a dependable and high throughput procedure well suited to routine applications. Iridoids were quantified in the range of 240–1140 ng with RSD of repeatability and intermediate precision between 0.9–2.5% and accuracy with bias 1.6–2.6%. The method was tested on six commercial Gardenia Jasminoides fruit samples